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The investigators hypothesized anticarcinogenic protective mechanisms of GSH to include its direct antioxidant function, indirect maintenance of other antioxidants, possible mediation of DNA synthesis and repair, and the ability to bind with cellular mutagens

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Cells in our in vitro experiments were stimulated with 5g/mL of (13)-B-D-glucan from Alcaligenes faecalis (Sigma-Aldrich), 100ng/mL of lipopolysaccharide (LPS) from Escherichia coli O127:B8 (Sigma-Aldrich), 120M L-buthionine sulfoximine (Sigma-Aldrich), and/or 1mg/mL of reduced glutathione (Sigma-Aldrich) for 24 hours unless otherwise stated in figure legends